| Category: |
Vesiculo-Bullous Disease |
|---|---|
| Test Code: | 104 |
| Disease: | Pemphigus/Pemphigoid/EBA |
| Methodology: | Indirect Immunofluorescence |
| Substrate: | Rodent bladder, Primate esophagus and Guinea Pig esophagus |
| Reference Range: | Negative: <1:10 |
| Units: | Titer |
| Note: | Positive samples at a 1:10 screening dilution are titered to an endpoint at an additional charge. |
| CPT Code: | 86255 |
| Schedule / Turnaround Time: | Assay performed daily Monday-Friday. Report availability is within 48 hours from the time of specimen receipt. |
| Specimen Requirements: |
Specimen need not be refrigerated or frozen. Collect 2-3 ml of blood in a red top or serum separator tube. If possible, separate serum from clot and place into white tube provided with Immco Diagnostics’ collection kits. If separation facilities are not available, the blood can be sent in the tube used for collection. |
| Sample Stability: |
Sample is stable at ambient temperature during shipment. If sample is stored prior to shipment, it is stable refrigerated (2-8˚C) up to five days and frozen (-20˚C or lower) up to one year. |
| Relevance: |
Paraneoplastic pemphigus is a form of pemphigus associated with malignancy. Positive paraneoplastic pemphigus sera give immunofluorescence reactions not only of pemphigus antibodies, but also react with other epithelia. Thus serum tests for this suspected condition are performed on an additional substrate. |