| Category: |
Gastroenterology & Hepatology Vesiculo-Bullous Disease |
|---|---|
| Test Code: | 100 |
| Disease: | • Celiac Disease (CD) • Dermatitis Herpetiformis (DH) |
| Methodology: | Indirect Immunofluorescence |
| Substrate: | Primate Smooth Muscle |
| Reference Range: | Negative: <1:2.5 |
| Units: | Titer |
| Note: | Positive samples at a 1:2.5 screening dilution are titered to an endpoint at an additional charge. |
| CPT Code: | 86255 |
| Schedule / Turnaround Time: | Assay performed daily Monday-Friday. Report availability is within 48 hours from the time of specimen receipt. |
| Specimen Requirements: |
Specimen need not be refrigerated or frozen. Collect 2-3 ml of blood in a red top or serum separator tube. If possible, separate serum from clot and place into white tube provided with Immco Diagnostics’ collection kits. If separation facilities are not available, the blood can be sent in the tube used for collection. |
| Sample Stability: |
Sample is stable at ambient temperature during shipment. If sample is stored prior to shipment, it is stable refrigerated (2-8˚C) up to five days and frozen (-20˚C or lower) up to one year. |
| Relevance: |
The detection of endomysial antibodies aids in the diagnosis of gluten sensitive enteropathy, i.e. celiac disease (CD) and dermatitis herpetiformis (DH). Patients with CD and DH are reported to have antibodies to endomysium, reticulin and gliadin. Of the various antibody markers of CD and DH, EMA of the IgA class seem to be the most sensitive and specific marker.Clinical Relevance: The detection of endomysial antibodies aids in the diagnosis of gluten sensitive enteropathy, i.e. celiac disease (CD) and dermatitis herpetiformis (DH). Patients with CD and DH are reported to have antibodies to endomysium, reticulin and gliadin. Of the various antibody markers of CD and DH, EMA of the IgA class seem to be the most sensitive and specific marker.Clinical Relevance: The detection of endomysial antibodies aids in the diagnosis of gluten sensitive enteropathy, i.e. celiac disease (CD) and dermatitis herpetiformis (DH). Patients with CD and DH are reported to have antibodies to endomysium, reticulin and gliadin. Of the various antibody markers of CD and DH, EMA of the IgA class seem to be the most sensitive and specific marker. |