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MPO (p-ANCA)
Technology : ELISA ~ Vasculitis & Glomerulonephritis Brand : CAPTIA™ ~ Vasculitis & Glomerulonephritis Analyte : Autoimmune ~ Liver - Kidney Disorders Intended UseThe Trinity Biotech Captia™ Myeloperoxidase (MPO) IgG, A, M Enzyme- LinkedImmunosorbent Assay (ELISA) is intended for the detection and semi- quantitation antibodies to myeloperoxidase in human sera. The assay is to be used to detect antibodies in a single serum specimen. The results of the assay are to be used as an aid to the diagnosis of microscopic polyangiitis. For in vitro diagnostic use. High complexity test. PrincipleThe Trinity Biotech MPO test is an Enzyme- Linked Immunosorbent Assay to detect IgG, IgA, and IgM antibodies to myeloperoxidase. Purified MPO antigens are attached to a solid phase microassay well. Enzyme- Linked Immunosorbent Assays (ELISA) rely on the ability of biological materials (i. e., antigens) to adsorb to plastic surfaces such as polystyrene (solid phase). When antigens bound to the solid phase are brought into contact with a patient's serum, antigen specific antibody, if present, will bind to the antigen on the solid phase forming antigen- antibody complexes. Excess antibody is removed by washing. This is followed by the addition of goat anti- human IgG, A, M conjugated with horseradish peroxidase which then binds to the antibody- antigen complexes. The excess conjugate is removed by washing, followed by the addition of Chromogen/ Substrate, tetramethylbenzidine (TMB). If specific antibody to the antigen is present in the patient's serum, a blue color develops. When the enzymatic reaction is stopped with 1N H2SO4 , the contents of the wells turn yellow. The color, which is indicative of the concentration of antibody in the serum, can be read on a suitable spectrophotometer or ELISA microwell plate readerProduct Code : 2338870 Format : CAPTIA™ 96 Well Test/Kit : 96 tests FDA : Cleared CE : Cleared Canadian Licensed: : Cleared Additional Information :
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